Description
Cedarlane"santi-ratCD45monoclonalantibodyrecognizesamonomorphicdeterminantoftheratleukocytecommonantigen(1).Theantigenrecognizedisaheavilyglycosylatedmembraneglycoproteinofmolecularweight170,000Daonthymocytesbutmolecularweight170,000-220,000Daonotherleukocytes.Theleukocytecommonantigen(L-CA)isamajorglycoproteinofhaematopoieticcellsbutisnotfoundonothertissuesorerythroidcells. Itispresentongreaterthan95%ofthymocytes,bonemarrowcellsandthoracicductlymphocytes.ThismoleculecarriesmuchofthecarbohydrateofthymocytesandshowsinterestingheterogeneityamongstTlymphocytesandBlymphocytes(2,3).Thisantibodyissuitableforuseinflowcytometryandimmunohistochemistrywithfrozensections.Thisclonehasalsobeenreportedtobeunsuitableforusewithparaffinsections.
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FlowCytometryAnalysis
Wistarratthymocyteswerestainedwithanti-CD45(clone:OX-30)(filledhistogram)ormouseIgG2aisotypecontrol(openhistogram).